Silhavy Lab

Silhavy Lab Members

Principal Investigator

Tom new headshot copy copy 
Thomas J. Silhavy, Ph.D.
Principal Investigator

Research Interest:  Protein targeting and signal transduction.
Thomas J. Silhavy Biography »

Thomas J. Silhavy CV pdf-icon-25x25


Postdoctoral Fellows


Marcin new
Marcin Grabowicz, Ph.D.

B.Sc. (Hons), Microbiology & Immunology
University of Adelaide (Australia), 2006

Ph.D., Microbiology & Immunology
University of Adelaide (Australia), 2010

Publications:

Lee, J., Xue, M., Wzorek, J.S., Wu, T., Grabowicz, M., Gronenberg, L.S., Sutterlin, H.A., Davis, R.M., Ruiz, N., Silhavy, T.J., Kahne, D. (2016). Barrel formation occurs in the periplasm before membrane insertion during folding on the β-barrel assembly machine. Proc Natl Acad Sci U S A. in press.

Grabowicz, M., Koren, D., Silhavy, T.J. (2016). The CpxQ sRNA negatively regulates Skp to prevent mistargeting of β-barrel outer membrane proteins into the cytoplasmic membrane. mBio 7 e00312-16.

Balibar CJ, Grabowicz, M. (2015). Mutant alleles of lptD increase the permeability of Pseudomonas aeruginosa and define determinants of intrinsic resistance to antibiotics. Antimicrob. Agents Chemother. 60:845-54.

Doyle, M.T., Grabowicz, M., Morona, R. (2015). A small conserved motif supports polarity augmentation of Shigella flexneri IcsA. Microbiology 161(11):2087-97.

Doyle, M.T., Grabowicz, M., May, K.L., Morona, R. (2015). Lipopolysaccharide surface structure does not influence IcsA polarity. FEMS Microbiol. Lett.. 362(8):fnv042.

Grabowicz, M., Andres, D., Lebar, M.D., Malojčić, G., Kahne, D., Silhavy, T.J. (2014). A mutant Escherichia coli that attaches peptidoglycan to lipopolysaccharide and displays cell wall on its surface. eLife 3:e05334.

Malojčić, G., Andres, D., Grabowicz, M., George, A.H., Ruiz, N., Silhavy, T.J., Kahne, D. (2014). LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface. Proc Natl Acad Sci U S A. 111(26):9467-72.

Grabowicz, M., Yeh, J., and T.J. Silhavy (2013). A dominant-negative lptE mutation that supports a role for LptE as a plug in the LptD barrel. J Bacteriol. 195: 1327–1334.

May, K.L.*, Grabowicz, M.*, Polyak, S.W., and R. Morona (2012). Self-association of the Shigella flexneri IcsA autotransporter protein. Microbiology 158:1874–1883. *authors contributed equally

Ogunniyi, A.D.*, M. Grabowicz*, L.K. Mahdi, J. Cook, D.L. Gordon, T. Sadlon, and J.C. Paton (2009) Pneumococcal histidine triad proteins are regulated by the Zn2+-dependent repressor AdcR and inhibit complement deposition through the recruitment of complement factor. H. FASEB J. 23:731-738. *Authors contributed equally to this manuscript

Ogunniyi, A.D., M. Grabowicz, D.E. Briles, J. Cook, and J.C. Paton (2007) Development of a vaccine against invasive pneumococcal disease based on combinations of virulence proteins of Streptococcus pneumoniae. Infect. Immun. 75: 350-357.


anna Anna Konovalova, Ph.D.

B.S. Biology/Microbiology, 2005
Taras Shevchenko National University of Kyiv, Ukraine

M.S. Microbiology and Virology, 2007
Taras Shevchenko National University of Kyiv, Ukraine

Ph.D. Microbiology, 2011
Max Planck Institute for terrestrial microbiology, Germany

Peer reviewed publications

Konovalova A., A.M. Mitchell, T.J. Silhavy (2016) An interlocked lipoprotein/β barrel complex monitors lipopolysaccharide integrity and transduces information across the outer membrane of Escherichia coli. eLife, 10.7554/eLife.15276.

Konovalova A., J. A. Schwalm, T.J. Silhavy (2016) A suppressor mutation that creates a faster and more robust σE envelope stress response. J. Bacteriology, online ahead of print.

Konovalova, A., D.H. Perlman, C.E. Cowles, T.J. Silhavy (2014) Transmembrane domain of surface-exposed outer membrane lipoprotein RcsF is threaded through the lumen of β-barrel proteins. Proc Natl Acad Sci U S A 111(41):E4350-4358.

Konovalova. A., S. Wegener-Feldbrügge & L. Søgaard-Andersen (2012) Two intercellular signals required for fruiting body formation in Myxococcus xanthus act sequentially but non-hierarchically. Mol Microbiol. 86(1):65-81.

Konovalova, A., S.Löbach & L. Søgaard-Andersen (2012). A RelA-dependent two-tiered regulated proteolysis cascade controls synthesis of a contact-dependent intercellular in Myxococcus xanthus. Mol. Microbiol. 84(2):260-75.

Kahnt, J., K. Aguiluz, J. Koch, A. Treuner-Lange, A. Konovalova, S. Huntley, M. Hoppert, L. Søgaard-Andersen & R. Hedderich, (2010) Profiling the outer membrane proteome during growth and development of the social bacterium Myxococcus xanthus by selective biotinylation and analyses of outer membrane vesicles. J Proteome Res 9: 5197-5208.

Rolbetzki, A., M. Ammon, V. Jakovljevic, A. Konovalova & L. Søgaard-Andersen, (2008) Regulated secretion of a protease activates intercellular signalling during fruiting body formation in M. xanthus. Dev Cell 15: 627-634.

Book chapter and reviews

Konovalova A., T.J. Silhavy (2015) Outer membrane lipoprotein biogenesis: Lol is not the end. Philos Trans R Soc Lond B Biol Sci 370 (1679).

Konovalova, A., L. Søgaard-Andersen & L. Kroos (2014) Regulated proteolysis in bacterial development. FEMS Microbiol Rev 38(3):493-522.

Konovalova, A. & L. Søgaard-Andersen (2011) Close encounters: Contact-dependent interactions in bacteria. Mol. Microbiol. 81: 297-301.

Konovalova, A., T. Petters & L. Søgaard-Andersen (2010) Extracellular biology of Myxococcus xanthus. FEMS Microbiol Rev 34: 89-106.


 

 Kerrie

Kerrie May, Ph.D.
kmay@princeton.edu

B.Biotechnology
University of Adelaide (Australia), 2001

B.Sc. (Hons), Microbiology & Immunology
University of Adelaide (Australia), 2002

Ph.D., Microbiology & Immunology
University of Adelaide (Australia), 2007

Publications:

May, K.L. and Silhavy, T.J. (2016) Making a membrane on the other side of the wall.  BBA Molecular and Cell Biology of Lipids. pii: S1388-1981(16)30272-4. 

Mashruwala, A.A., Roberts, C.A., Bhatt, S., May, K.L., Carroll, R.K., Lindsey N. Shaw, L.N. and Boyd, J.M. (2016). Staphylococcus aureus SufT: an essential iron-sulfur cluster assembly factor in cells experiencing a high-demand for lipoic acid. Molecular Microbiology.  102(6):1099–1119

Sutterlin, H.A.*, Shi, H.*, May, K.L., Miguel, A., Khare, S., Huang, K.C. and Silhavy, T.J. (2016) Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway. Proceedings of the National Academy of Sciences. 113(11): E1565-74 *authors contributed equally

Basu, D., Li, X.P., Kahn, J.N., May, K.L., Kahn P.C. and Tumer, N.E. (2015) The A1 subunit of Shiga toxin 2 has higher affinity for ribosomes and higher catalytic activity than the A1 subunit Shiga toxin 1. Infection and Immunity.  84(1):149-61

Doyle, M., Grabowicz, M., May, K.L. and Morona R. (2015) Lipopolysaccharide surface structure does not influence IcsA polarity. FEMS Microbiology Letters.  362(8): fnv042.

May, K.L., Yan, Q and Tumer, N.E. (2013) Targeting ricin to the ribosome. Toxicon. 69:143-51. 

May, K.L., Li, X.P., Martínez-Azorín, F., Ballesta, J.P.G., Grela, P., Tchórzewski, M and Tumer, N.E. (2012) The P1/P2 proteins of the human ribosomal stalk are required for ribosome binding and depurination by ricin in human cells. FEBS Journal. 279:3925–3936

May, K.L.*, Grabowicz, M.*, Polyak, S. and Morona, R. (2012) Self-association of the Shigella flexneri IcsA autotransporter protein. Microbiology. 158(7):1874-83. *authors contributed equally

May, K.L., Paton, J.C., Paton, A.W. (2010) Escherichia coli subtilase cytotoxin induces apoptosis regulated by host Bcl-2 family proteins Bax/Bak. Infection and Immunity. 78(11):4691-6.

Wolfson, J.J., May, K.L., Thorpe, C.M., Jandhyala, D.M., Paton, J.C., Paton, A.W. (2008) Subtilase cytotoxin activates PERK, IRE1, and ATF6 endoplasmic reticulum stress-signaling pathways. Cellular Microbiology. 10(9):1775-1786. 

May, K.L. and Morona, R. (2008) Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host N-WASP. Journal of Bacteriology. 190(13):4666-4676.
 
 
angela 
Angela Mitchell, Ph.D.

B.S. in Biotechnology, Summa cum Laude, Honors in the Discipline
Elizabethtown College, 2008
Ph.D. in Microbiology and Immunology
University of North Carolina at Chapel Hill, 2013

Publications:

Mitchell AM, Wang W, Silhavy TJ (2017). Novel RpoS-dependent mechanisms strengthen the envelope permeability barrier during stationary phase. Journal of Bacteriology. 199(2): e00708-16.

Konovalova A, Mitchell AM, & Silhavy TJ (2016). A lipoprotein/β-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane. eLife. 10.7554/eLife.15276.

Xiao PJ, Mitchell AM, Haung L, Li C, & Samulski RJ (2016). Disruption of microtubules post virus entry enhances adeno-associated virus vector transduction. Human Gene Therapy. 27(4):309-24.

Mitchell AM, Moser R, Samulski RJ, & Hirsch ML. (2016) Stimulation of AAV gene editing via DSB repair. In Hirsch ML, Cathomen T, & Porteus MH (Eds.), Genome Editing: The Next Step in Gene Therapy (pgs. 125-137). ISBN: 978-1-4939-3507-9.

Vance MA, Mitchell AM, & Samulski RJ. (2015) rAAV biology, infectivity and therapeutic use from bench to clinic. In Hashad ID (Ed.), Gene Therapy. ISBN: 978-953-51-4380-2Mitchell AM, Hirsch ML, Li C, & Samulski RJ (2014). The promyelocytic leukemia protein is a cell-intrinsic defense factor inhibiting parvovirus DNA replication. Journal of Virology. 88(2): 925-36.

Mitchell AM & Samulski RJ (2013). Mechanistic insights into the enhancement of adeno-associated virus transduction by proteasome inhibitors. Journal of Virology. 87(23): 13035-41.

Mitchell AM, Li C, & Samulski RJ (2013). Arsenic trioxide stabilizes accumulations of adeno-associated virus virions at the perinuclear region, increasing transduction in vitro and in vivo. Journal of Virology. 87(8): 4571-83.

Mitchell AM*, Nicolson SC*, Warischalk JK*, & Samulski RJ (2010). AAV’s Anatomy: Roadmap for Optimizing Vectors for Translational Success. Current Gene Therapy. 10(5): 319-340. *These authors contributed equally to this manuscript.
 

   
   
   

Graduate Students


 JGrimm
 Jackie Grimm
jgrimm@princeton.edu

B.S., Biology
University of Virginia, 2012

 
 
 BHart
Betsy Hart
 
B.A. in Anthropology and Biology (Honors)
Williams College, 2014

 
Anne McCabe
 
A.B, Molecular and Cellular Biology
Harvard College, 2011