Ploss Lab Publications


Li D, Wang X, von Schaewen M, Tao W, Zhang Y, Heller B, Hrebikova G, Deng Q, Sun Q, Ploss A, Zhong J, Huang Z. 2017. Immunization with a subunit hepatitis C virus vaccine elicits pan-genotypic neutralizing antibodies and intra-hepatic T-cell responses in non-human primates. The Journal of infectious diseases.

Shirvani-Dastgerdi E, Winer BY, Celia-Terrassa T, Kang Y, Tabernero D, Yagmur E, Rodriguez-Frias F, Gregori J, Luedde T, Trautwein C, Ploss A, Tacke F. 2017. Selection of the highly replicative and partially multidrug resistant rtS78T HBV polymerase mutation during TDF-ETV combination therapy. Journal of hepatology.

Douam F, Hrebikova G, Albrecht YE, Sellau J, Sharon Y, Ding Q, Ploss A. 2017. Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome. Nature communications 8:14781.

Ding Q, Heller B, Capuccino JM, Song B, Nimgaonkar I, Hrebikova G, Contreras JE, Ploss A. 2017. Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles. Proceedings of the National Academy of Sciences of the United States of America.


Winer BY, Huang T, Low BE, Avery C, Pais MA, Hrebikova G, Siu E, Chiriboga L, Wiles MV, Ploss A. 2016. Recapitulation of treatment response patterns in a novel humanized mouse model for chronic hepatitis B virus infection. Virology 502:63-72.

Ding Q, von Schaewen M, Hrebikova G, Heller B, Sandmann L, Plaas M, Ploss A. 2016. Mice expressing minimally humanized CD81 and occludin genes support hepatitis C virus uptake in vivo. Journal of virology.

von Schaewen M, Dorner M, Hueging K, Foquet L, Gerges S, Hrebikova G, Heller B, Bitzegeio J, Doerrbecker J, Horwitz JA, Gerold G, Suerbaum S, Rice CM, Meuleman P, Pietschmann T, Ploss A. 2016. Expanding the Host Range of Hepatitis C Virus through Viral Adaptation. mBio 7.

Li D, von Schaewen M, Wang X, Tao W, Zhang Y, Li L, Heller B, Hrebikova G, Deng Q, Ploss A, Zhong J, Huang Z. 2016. Altered Glycosylation Patterns Increase Immunogenicity of a Subunit Hepatitis C Virus Vaccine, Inducing Neutralizing Antibodies Which Confer Protection in Mice. Journal of virology 90:10486-10498.

Shirvani-Dastgerdi E, Schwartz RE, Ploss A. 2016. Hepatocarcinogenesis associated with hepatitis B, delta and C viruses. Current opinion in virology 20:1-10.

Basta NE, Mahmoud AA, Wolfson J, Ploss A, Heller BL, Hanna S, Johnsen P, Izzo R, Grenfell BT, Findlow J, Bai X, Borrow R. 2016. Immunogenicity of a Meningococcal B Vaccine during a University Outbreak. The New England journal of medicine 375:220-228.

Bartucci M, Ferrari AC, Kim IY, Ploss A, Yarmush M, Sabaawy HE. 2016. Personalized Medicine Approaches in Prostate Cancer Employing Patient Derived 3D Organoids and Humanized Mice. Frontiers in cell and developmental biology 4:64.

von Schaewen, M., Hrebikova, G. & Ploss, A. (2016). Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens. Methods Mol Biol 1438, 79-101.

Winer. B.Y., Ding, Q., Gaska, J., Ploss, A. (2016). In vivo model of hepatitis B and C virus infection. FEBS Lett. 590:1987-1999.

Douam, F., Ding, Q., Ploss, A. (2016). Recent Advances in Understanding Hepatitis C. F1000Reseach 5(F1000 Faculty Rev):131.

Winer, B.Y., and Ploss, A. (2016). Breaking the species barrier for hepatitis delta virus. Hepatology 63, 334-336.

Perin, P.M., Haid, S., Brown, R.J., Doerrbecker, J., Schulze, K., Zeilinger, C., von Schaewen, M., Heller, B., Vercauteren, K., Luxenburger, E., et al. (2016). Flunarizine prevents hepatitis C virus membrane fusion in a genotype-dependent manner by targeting the potential fusion peptide within E1. Hepatology 63, 49-62.


Douam, F., Gaska, J.M., Winer, B.Y., Ding, Q., von Schaewen, M., and Ploss, A. (2015). Genetic Dissection of the Host Tropism of Human-Tropic Pathogens. Annu Rev Genet 49, 21-45.

Ploss, A., and Walker, C. (2015). Editorial overview: Progress and challenges in modeling human viral diseases in vivo. Current opinion in virology 13, v-vii.

Winer, B.Y., and Ploss, A. (2015). Determinants of hepatitis B and delta virus host tropism. Current opinion in virology 13, 109-116.

Scull, M.A., Shi, C., de Jong, Y.P., Gerold, G., Ries, M., von Schaewen, M., Donovan, B.M., Labitt, R.N., Horwitz, J.A., Gaska, J.M., et al. (2015). Hepatitis C virus infects rhesus macaque hepatocytes and simianized mice. Hepatology 62, 57-67.

Douam, F., and Ploss, A. (2015). Proteomic approaches to analyzing hepatitis C virus biology. Proteomics 15, 2051-2065.

Stoddard, M.B., Li, H., Wang, S., Saeed, M., Andrus, L., Ding, W., Jiang, X., Learn, G.H., von Schaewen, M., Wen, J., et al. (2015). Identification, molecular cloning, and analysis of full-length hepatitis C virus transmitted/founder genotypes 1, 3, and 4. mBio 6, e02518.

Gardner, M.R., Kattenhorn, L.M., Kondur, H.R., von Schaewen, M., Dorfman, T., Chiang, J.J., Haworth, K.G., Decker, J.M., Alpert, M.D., Bailey, C.C., et al. (2015). AAV-expressed eCD4-Ig provides durable protection from multiple SHIV challenges. Nature 519, 87-91.

Siu, E., and Ploss, A. (2015). Modeling malaria in humanized mice: opportunities and challenges. Ann N Y Acad Sci 1342, 29-36.

Gaska, J.M., and Ploss, A. (2015). Study of viral pathogenesis in humanized mice. Current opinion in virology 11, 14-20.


Ding, Q., von Schaewen, M., and Ploss, A. (2014). The impact of hepatitis C virus entry on viral tropism. Cell Host Microbe 16, 562-568.

Lacek, K., Urbanowicz, R.A., Troise, F., De Lorenzo, C., Severino, V., Di Maro, A., Tarr, A.W., Ferrara, F., Ploss, A., Temperton, N., et al. (2014). Dramatic potentiation of the antiviral activity of HIV antibodies by cholesterol conjugation. J Biol Chem 289, 35015-35028.

de Jong YP, Dorner M, Mommersteeg MC, Xiao JW, Balazs AB, Robbins JB, Winer BY, Gerges S, Vega K, Labitt RN, Donovan BM, Giang E, Krishnan A, Chiriboga L, Charlton MR, Burton DR, Baltimore D, Law M, Rice CM, Ploss A. (2014) Broadly neutralizing antibodies abrogate established hepatitis C virus infection. Sci Transl Med. 6(254):254ra129.

Ploss A. (2014) Mouse models for human infectious diseases. J Immunol Methods. Jul 11. [Epub ahead of print]

Billerbeck E, Labitt RN, Vega K, Frias-Staheli N, Dorner M, Xiao J, Rice CM, Ploss A. (2014) Insufficient IL-12 signaling favors differentiation of human CD4+ and CD8+ T cells into GATA-3+ and GATA-3+ T-bet+ subsets in humanized mice. Immunology. Apr 25. [Epub ahead of print]

von Schaewen M, Ploss A. (2014) Murine models of hepatitis C: What can we look forward to? Antiviral Res. 104: 15-22. Pubmed


Horwitz JA, Halper-Stromberg A, Mouquet H,...Ploss A,...Nussenzweig MC. (2013) HIV-1 suppression and durable control by combining single broadly neutralizing antibodies and antiretroviral drugs in humanized mice. Proc Natl Acad Sci. 110: 16538-43. Pubmed

Anggakusuma, Colpitts CC, Schang LM,...Ploss A, Pietschmann T, Steinmann E. (2013) Turmeric curcumin inhibits entry of all hepatitis C virus genotypes into human liver cells. Gut. [Epub ahead of print]

Dorner M, Horwitz JA, Donovan BM,...Ploss A. (2013) Completion of the entire hepatitis C virus life cycle in genetically humanized mice. Nature. 501: 237-41. Pubmed

Billerbeck E, Horwitz JA, Labitt RN,...Ploss A. (2013) Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol. 191: 1753-64.  Pubmed

Gruell H, Bournazos S, Ravetch JV, Ploss A, Nussenzweig MC, Pietzsch J. (2013) Antibody and antiretroviral pre-exposure prophylaxis prevent cervicovaginal HIV-1 infection in a transgenic mouse model. J Virol. 87: 8535-44. Pubmed

Vogt A, Scull MA, Friling T...Ploss A. (2013) Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines. Virology. 444: 1-11. Pubmed

Guermonprez P, Helft J, Claser C,...Ploss A,...Nussenzweig MC. (2013) Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection. Nat Med. 19: 730-38. Pubmed

Horwitz JA, Dorner M, Friling T,...Ploss A. (2013) Expression of heterologous proteins flanked by NS3-4A cleavage sites within the hepatitis C virus polyprotein. Virology. 439: 23-33.  Pubmed

Billerbeck E, de Jong Y, Dorner M, de la Fuente C, Ploss A. (2013) Animal models for hepatitis C. Curr Top Microbiol Immunol. 369: 49-86. Pubmed

Shi C, Ploss A. (2013) Hepatitis C virus vaccines in the era of new direct-acting antivirals. Expert Rev Gastroenterol Hepatol. 7: 171-85. Pubmed

Sandmann L, Ploss A. (2013) Barriers of hepatitis C virus interspecies transmission. Virology. 435: 70-80. Pubmed

Dorner M, Rice CM, Ploss A. (2013) Study of hepatitis C virus entry in genetically humanized mice. Methods. 59: 249-57. Pubmed


Klein F, Halper-Stromberg A, Horwitz JA...Ploss A, Nussenzweig MC. (2012), HIV therapy by a combination of broadly neutralizing antibodies in humanized mice, Nature. 492: 118-22.

Pietzsch J, Gruell H, Bournazos S...Ploss A, Nussenzweig MC. (2012) A mouse model for HIV entry. Proc Natl Acad Sci. 109: 15859-64.

Vaughan AM, Mikolajczak SA, Wilson EM...Ploss A, Kappe SH. (2012) Complete Plasmodium falciparum liver-stage development in liver-chimeric mice. J Clin Invest. 122: 3618-28.

Schoggins JW, Dorner M, Feulner M, Imanaka N, Murphy MY, Ploss A, Rice CM. (2012) Development of fully infectious dengue reporter viruses for bioluminescent imaging and high throughput screening, Proc Natl Acad Sci. 109: 14610-5.

Vaughan AM, Kappe SH, Ploss A, Mikolajczak SA. (2012) Development of humanized mouse models to study human malaria parasite infection. Future Microbiol. 7: 657-65.

Ploss A*, Dubuisson J.* (2012) New advances in the molecular biology of hepatitis C virus infection: towards the identification of new treatment targets, Gut. 61 Suppl 1:i25-i35.

Giang E, Dorner M, Prentoe JC...Ploss A, Burton DR, Law M. (2012) Human broadly neutralizing antibodies to the envelope glycoprotein complex of hepatitis C virus. Proc Natl Acad Sci. 109: 6205-10.

Ploss A*, Evans M.* (2012) Hepatitis C virus entry. Curr Opin Virol. 2: 14-9.

Meng X, Schoggins J, Rose L...Ploss A...Xiang Y. (2012) C7L family of poxvirus host range genes inhibits antiviral activities induced by type I interferons and interferon regulatory factor 1. J Virology. 86: 4538-47.

Schwartz RE*, Trehan K*, Andrus L, Ploss A...Bhatia SN. (2012) Modeling hepatitis C virus infection using human induced pluripotent stem cells. Proc Natl Acad Sci. 109: 2544-8.

Scull MA, Ploss A. (2012) Exiting from uncharted territory: Hepatitis C virus assembles in mouse cell lines. Hepatology. 55: 645-8.


Dorner M, Ploss A. (2011) Deconstructing hepatitis C virus infection in small animal models. (2011) Ann N Y Acad Sci. 1245:59-62.

Marukian S, Andrus L, Sheahan TP, Charles ED, Ploss A, Rice CM, Dustin LB. (2011) Hepatitis C virus induces interferon-λ and interferon-stimulated genes in primary liver cultures. Hepatology. 54: 1913-23.

Andrus L, Marukian S, Jones CT...Ploss A...Rice CM. (2011) Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells. Hepatology. 54: 1901-12.

Dorner M, Horwitz JA, Robbins J...Ploss A. (2011) A genetically humanized mouse model for hepatitis C virus infection. Nature. 474: 208-11

Washburn ML, Bility MT, Kovalev GI...Ploss A...Su L. (2011) A novel humanized mouse model with a human immune system and human liver supports Hepatitis C virus infection, immune responses, and hepatopathogenesis. Gastroenterology. 40: 1334-44.

Billerbeck E, Barry WT, Mu K, Dorner M, Rice CM, Ploss A. (2011) Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor, GM-CSF and interleukin 3 expressing NOD SCID IL2RγNULL humanized mice. Blood. 117: 3076-86.


De Jong YP, Rice CM, Ploss A. (2010) Evaluation of combination therapy against hepatitis C virus infection in human liver chimeric mice. J. Hepatol. 54: 848-50.

Gerold G, Rice CM, Ploss A. (2010) Teaching new tricks to an old foe: murinizing Hepatitis C virus, Hepatology 52: 2233-6.

Sheahan T, Jones CT, Ploss A. (2010) Advances and challenges in studying hepatitis C virus in its native environment, Expert Rev Gastroenterol Hepatol. 4: 541-50.

Kohaar I, Ploss A, Korol E...Prokunina-Olsson L. (2010) Splicing diversity of human OCLN gene and its biological significance for hepatitis C virus (HCV) entry. J Virol. 84: 6987-94.

De Jong YP, Rice CM, Ploss A. (2010) New horizons for studying human hepatotropic infections. J Clin Invest. 120: 650-3.

Jones CT, Catanese MT, Law LMJ...Ploss A...Rice CM. (2010) Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system. Nat Biotechnol. 28: 167-71.

Ploss A*, Khetani SK*, Jones CT...Bhatia SN. (2010) Persistent hepatitis C virus infection in microscale primary human hepatocyte cultures. Proc Natl Acad Sci. 107: 3141-5.


 Akondy RS, Monson ND, Miller JD...Ploss A...Ahmed R. (2009) The yellow fever virus vaccine induces a broad and polyfunctional human memory CD8+ T cell response. J Immunol. 183: 7919-30.

Ploss A and Rice CM. (2009) Towards a small model for Hepatitis C. EMBO Rep. 10: 1220-7.

Legrand N*, Ploss A*, Balling R...Ziegler P. (2009) Humanized mice for modeling human infectious disease: challenges, progress, and outlook. Cell Host Microbe. 6: 5-9.

Strowig T*, Gurer C*, Ploss A...Münz C. (2009) Priming of protective T cell responses against virus-induced tumors in mice with human immune system components. J Exp Med. 206: 1423-34.

Ploss A*, Evans MJ*, Gaysinskaya VA...Rice CM. (2009) Human occludin is a hepatitis C virus entry factor required for infection of mouse cells, Nature 457: 882-6.

Biswas PS, Pedicord V, Ploss A, Menet E, Leiner I, Pamer EG. (2007) Pathogen-specific CD8 T cell responses are directly inhibited by IL-10. J Immunol. 179: 4520-8.

Lindenbach BD, Meuleman P, Ploss A...Rice CM. (2006) Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro. Proc Natl Acad Sci. 103: 3805-9.

Ploss A, Leiner I, Pamer EG. (2005) Distinct regulation of H2-M3 restricted memory T cell responses in lymph node and spleen. J Immunol. 175: 5998-6005.

Ploss A, Tran A, Menet E, Leiner I, Pamer EG. (2005) Cross-recognition of N-formyl methionine peptides is a general characteristic of H2-M3 restricted CD8+ T cells. Infect Immun. 73: 4423-6.

Dao T, Guo D, Ploss A...Sant'Angelo DB. (2004) Development of CD1d-restricted NKT cells in the mouse thymus. Eur J Immunol. 34: 3542-52.

Wong P, Lara-Tejero M, Ploss A, Leiner I, Pamer EG. (2004) Rapid development of T cell memory. J Immunol. 172: 7239-45.

Ploss A, Lauvau G, Contos B...Pamer EG. (2003) Promiscuity of MHC class Ib restricted T cell responses. J Immunol. 171: 5948-55.

Kerksiek KM, Ploss A, Leiner I, Busch DH, Pamer EG. (2003) H2-M3 restricted T cells respond to secondary antigen exposure. J Immunol. 170: 1862-9.

Reuss FU, Heber R, Ploss A, Berdel B. (2001) Amphotropic murine leukemia virus replication in human mammary epithelial cells and the formation of cytomegalovirus-promoter recombinants. Virology. 291: 91-100.

Book Chapters

Ploss A. (2012) Hepatitis C virus and use of reverse genetics in drug design, in A. Bridgen (Ed.) Chapter 3: Reverse Genetics of RNA Viruses: Applications and Perspectives, pages 64-90, Wiley-Blackwell

Ploss A, Pamer EG. (2005) Immunologic Memory. In: Meyers, R.A., ed. Encyclopedia of Molecular Cell Biology and Molecular Medicine, WILEY-VCH Verlag GmbH & Co., Weinheim. p. 383

Ploss A, Pamer EG. (2004) Memory, in S.H.E. Kaufmann (Ed.) Novel Vaccination Strategies, WILEY-VCH, Weinheim, New York, pp.73