Hughson Lab

Hughson Lab Publications

Recent Publications

Ha, J.Y., Chou, H.T., Ungar, D., Yip, C.K., Walz, T., and Hughson, F.M. (2016) Molecular architecture of the complete COG tethering complex. Nat Struct Mol Biol 23, 758-760.

Baker, R.W. and Hughson, F.M. (2016) Chaperoning SNARE assembly and disassembly. Nat Rev Mol Cell Biol 17, 465-479.

Boyaci, H., Shah, T., Hurley, A., Kokona, B., Li, Z., Ventocilla, C., Jeffrey, P.D., Semmelhack, M.F., Fairman, R., Bassler, B.L., and Hughson, F.M. (2016) Structure, regulation, and inhibition of the quorum-sensing signal integrator LuxO. PLoS Biol 14, e1002464.

Suckling, R.J., Poon, P.P., Travis, S.M., Hughson, F.M., Evans, P.R., Duden, R., and Owen, D.J. (2015) Structural basis for the binding of tryptophan-based motifs by delta-COP. Proc Natl Acad Sci USA 112, 14242-14247.

Baker RW, Jeffrey PD, Zick M, Phillips BP, Wickner WT, Hughson FM. (2015) A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science 349(6252): 1111-4.

Rogers JV, McMahon C, Baryshnikova A, Hughson FM, Rose MD. (2014) ER-associated retrograde SNAREs and the Dsl1 complex mediate an alternative, Sey1p-independent homotypic ER fusion pathway. Mol Biol Cell. 25: 3401-12.

Ha JY, Pokrovskaya ID, Climer LK,...Hughson FM. (2014) Cog5-Cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex. Proc Natl Acad Sci. 111: 15762-7.

Bharucha N, Liu Y, Papanikou E,...Hughson FM, Glick BS. (2013) Sec16 influences transitional ER sites by regulating rather than organizing COPII. Mol Biol Cell. 24: 3406-19.

Baker R.W., Jeffrey P.D., Hughson F.M. (2013) Crystal Structures of the Sec1/Munc18 (SM) Protein Vps33, Alone and Bound to the Homotypic Fusion and Vacuolar Protein Sorting (HOPS) Subunit Vps16 Plos One 2013 Jun 26;8(6):e67409.

Hughson FM. (2013) Chaperones that SNARE neurotransmitter release. Science. 339: 406-07.

McMahon C., Studer S.M., Clendinen C., Dann G.P., Jeffrey P.D., Hughson F.M. (2012) The structure of Sec12 implicates potassium ion coordination in Sar1 activation. J Biol Chem. 2012 Dec 21;287(52):43599-606.

Chen G., Swem L.R., Swem D.L., Stauff D.L., O'Loughlin C.T., Jeffrey P.D., Bassler B.L., Hughson F.M. (2011) A strategy for antagonizing quorum sensing. Mol Cell. 42, 199-209.


Lees, J.A., Yip, C.K., Walz, T., and Hughson, F.M. (2010) Molecular organization of the COG vesicle tethering complex. Nat. Struct. Mol. Biol. 17, 1292-7.

Yu, I.M., and Hughson, F.M. (2010) Tethering factors as organizers of intracellular vesicular traffic. Annu. Rev. Cell. Dev. Biol. 26, 137-156.

Hughson, F.M., and Reinisch, K.M. (2010) Structure and mechanism in membrane trafficking. Curr Opin Cell Biol. 22, 454-460.

Ren, Y., Yip, C.K., Tripathi, A., Huie, D., Jeffrey, P.D., Walz, T., and Hughson F.M. (2009) A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell 139, 1119-1129.

Kelly, R.C., Bolitho, M.E., Higgins, D.A., Lu, W., Ng, W.L., Jeffrey, P.D., Rabinowitz, J.D., Semmelhack, M.F., Hughson, F.M., and Bassler, B.L. (2009) The Vibrio cholerae quorum-sensing autoinducer CAI-1: analysis of the biosynthetic enzyme CqsA. Nat. Chem. Biol. 5, 891-895.

Richardson, B.C., Smith, R.D., Ungar, D., Nakamura, A., Jeffrey, P.D., Lupashin, V.V., and Hughson, F.M. (2009) Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene. Proc. Natl. Acad. Sci. U.S.A. 106, 13329-13334

Tripathi, A., Ren, Y., Jeffrey, P.D., and Hughson, F.M. (2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature Struct. Molec. Biol. 16, 114-123.

Hughson, F.M. (2008) Both layers of the COPII coat come into view [Preview]. Cell 134, 384-385.

Cavanaugh, L.F., Chen, X., Richardson, B.C., Ungar, D., Pelczer, I., Rizo, J., and Hughson, F.M. (2007)Structural analysis of conserved oligomeric Golgi complex subunit 2. J. Biol. Chem. 282, 23418-23426.

Neiditch, M.B. and Hughson, F.M. (2007) The regulation of histidine sensor kinase complexes by quorum sensing signal molecules. Meth. Enzymol. 423, 250-263.

Neiditch, M.B., Federle, M.J., Pompeani, A.J., Kelly, R.C., Swem, D.L., Jeffrey, P.D., Bassler, B.L., and Hughson, F.M. (2006) Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell 126, 1095-1108.

Togneri, J., Cheng, Y.S., Munson, M., Hughson, F.M., and Carr, C.M. (2006) SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p. Proc. Natl. Acad. Sci. USA 103, 17730-17735.

Ungar, D., Oka, T., Krieger, M., and Hughson, F.M. (2006) Retrograde transport on the COG railway [review] Trends Cell Biol. 16, 113-120.

Oka, T., Vasile, E., Penman, M., Novina, C.D., Dykxhoorn, D.M., Ungar, D., Hughson, F.M., Krieger, M. (2005) Genetic analysis of the subunit organization and function of the COG complex: Studies of Cog5 and Cog7 deficient mammalian cells. J. Biol. Chem. 280, 32736-32745.

Ungar, D., Oka, T., Vasile, E., Krieger, M., and Hughson, F.M. (2005) Subunit architecture of the conserved oligomeric Golgi complex. J. Biol. Chem. 280, 32729-32735.

Neiditch, M.B., Federle, M.J., Miller, S.T., Bassler, B.L., and Hughson, F.M. (2005) Regulation of LuxPQ receptor activity by the quorum sensing signal autoinducer-2. Mol. Cell 18, 507-518.