Enquist Lab Media

Live cell imaging of mitochondria and PRV capsids in axons of infected SCG neurons

SCG neurons were infected with PRV GS443, a wild type strain that expresses GFP-tagged capsids (GFP-VP26) and stained with 10 nM MitoTracker Red CMX Ros. PRV capsids (green) and mitochondria (red) were visualized in axons at 12 hpi on a Leica SP5 confocal microscope equipped for imaging multiple channels simultaneously. Images were captured at a rate of 1.2 frames/second for 180 seconds.